Found in All Human DNA
This was important enough that I wanted to get it out immediately. My research into the NCBI database for nucleotide sequences has lead to a stunning discovery. One of the WHO primer sequences in the PCR test for SARS-CoV-2 is found in all human DNA!
The sequence “CTCCCTTTGTTGTGTTGT” is an 18-character primer sequence found in the WHO coronavirus PCR testing protocol document. The primer sequences are what get amplified by the PCR process in order to be detected and designated a “positive” test result. It just so happens this exact same 18-character sequence, verbatim, is also found on Homo sapiens chromosome 8! As far as I can tell, this means that the WHO test kits should find a positive result in all humans. Can anyone explain this otherwise?
I really cannot overstate the significance of this finding. At minimum, it should have a notable impact on test results.
Homo sapiens chromosome 8, GRCh38.p12 Primary Assembly
Sequence ID: NC_000008.11 Length: 145138636
Range 1: 63648346 to 63648363 is “CTCCCTTTGTTGTGTTGT”
Update: After some effort, I have finally discovered a way to display proof (beyond my screenshots) that human chromosome 8 has this exact same 18-character sequence. Please try the link below. The sequence is shown at the bottom of the page.
Homo sapiens chromosome 8, GRCh38.p13 Primary Assembly
NCBI Reference Sequence: NC_000008.11
LOCUS NC_000008 18 bp DNA linear CON 17-AUG-2020 DEFINITION Homo sapiens chromosome 8, GRCh38.p13 Primary Assembly. ACCESSION NC_000008 REGION: 63648346..63648363 VERSION NC_000008.11 DBLINK BioProject: PRJNA168 Assembly: GCF_000001405.39 KEYWORDS RefSeq. SOURCE Homo sapiens (human) ORGANISM Homo sapiens Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. REFERENCE 1 (bases 1 to 18) AUTHORS Nusbaum,C., Mikkelsen,T.S., Zody,M.C., Asakawa,S., Taudien,S., Garber,M., Kodira,C.D., Schueler,M.G., Shimizu,A., Whittaker,C.A., Chang,J.L., Cuomo,C.A., Dewar,K., FitzGerald,M.G., Yang,X., Allen,N.R., Anderson,S., Asakawa,T., Blechschmidt,K., Bloom,T., Borowsky,M.L., Butler,J., Cook,A., Corum,B., DeArellano,K., DeCaprio,D., Dooley,K.T., Dorris,L. III, Engels,R., Glockner,G., Hafez,N., Hagopian,D.S., Hall,J.L., Ishikawa,S.K., Jaffe,D.B., Kamat,A., Kudoh,J., Lehmann,R., Lokitsang,T., Macdonald,P., Major,J.E., Matthews,C.D., Mauceli,E., Menzel,U., Mihalev,A.H., Minoshima,S., Murayama,Y., Naylor,J.W., Nicol,R., Nguyen,C., O'Leary,S.B., O'Neill,K., Parker,S.C., Polley,A., Raymond,C.K., Reichwald,K., Rodriguez,J., Sasaki,T., Schilhabel,M., Siddiqui,R., Smith,C.L., Sneddon,T.P., Talamas,J.A., Tenzin,P., Topham,K., Venkataraman,V., Wen,G., Yamazaki,S., Young,S.K., Zeng,Q., Zimmer,A.R., Rosenthal,A., Birren,B.W., Platzer,M., Shimizu,N. and Lander,E.S. TITLE DNA sequence and analysis of human chromosome 8 JOURNAL Nature 439 (7074), 331-335 (2006) PUBMED 16421571 REFERENCE 2 (bases 1 to 18) CONSRTM International Human Genome Sequencing Consortium TITLE Finishing the euchromatic sequence of the human genome JOURNAL Nature 431 (7011), 931-945 (2004) PUBMED 15496913 REFERENCE 3 (bases 1 to 18) AUTHORS Lander,E.S., Linton,L.M., Birren,B., Nusbaum,C., Zody,M.C., Baldwin,J., Devon,K., Dewar,K., Doyle,M., FitzHugh,W., Funke,R., Gage,D., Harris,K., Heaford,A., Howland,J., Kann,L., Lehoczky,J., LeVine,R., McEwan,P., McKernan,K., Meldrim,J., Mesirov,J.P., Miranda,C., Morris,W., Naylor,J., Raymond,C., Rosetti,M., Santos,R., Sheridan,A., Sougnez,C., Stange-Thomann,N., Stojanovic,N., Subramanian,A., Wyman,D., Rogers,J., Sulston,J., Ainscough,R., Beck,S., Bentley,D., Burton,J., Clee,C., Carter,N., Coulson,A., Deadman,R., Deloukas,P., Dunham,A., Dunham,I., Durbin,R., French,L., Grafham,D., Gregory,S., Hubbard,T., Humphray,S., Hunt,A., Jones,M., Lloyd,C., McMurray,A., Matthews,L., Mercer,S., Milne,S., Mullikin,J.C., Mungall,A., Plumb,R., Ross,M., Shownkeen,R., Sims,S., Waterston,R.H., Wilson,R.K., Hillier,L.W., McPherson,J.D., Marra,M.A., Mardis,E.R., Fulton,L.A., Chinwalla,A.T., Pepin,K.H., Gish,W.R., Chissoe,S.L., Wendl,M.C., Delehaunty,K.D., Miner,T.L., Delehaunty,A., Kramer,J.B., Cook,L.L., Fulton,R.S., Johnson,D.L., Minx,P.J., Clifton,S.W., Hawkins,T., Branscomb,E., Predki,P., Richardson,P., Wenning,S., Slezak,T., Doggett,N., Cheng,J.F., Olsen,A., Lucas,S., Elkin,C., Uberbacher,E., Frazier,M., Gibbs,R.A., Muzny,D.M., Scherer,S.E., Bouck,J.B., Sodergren,E.J., Worley,K.C., Rives,C.M., Gorrell,J.H., Metzker,M.L., Naylor,S.L., Kucherlapati,R.S., Nelson,D.L., Weinstock,G.M., Sakaki,Y., Fujiyama,A., Hattori,M., Yada,T., Toyoda,A., Itoh,T., Kawagoe,C., Watanabe,H., Totoki,Y., Taylor,T., Weissenbach,J., Heilig,R., Saurin,W., Artiguenave,F., Brottier,P., Bruls,T., Pelletier,E., Robert,C., Wincker,P., Smith,D.R., Doucette-Stamm,L., Rubenfield,M., Weinstock,K., Lee,H.M., Dubois,J., Rosenthal,A., Platzer,M., Nyakatura,G., Taudien,S., Rump,A., Yang,H., Yu,J., Wang,J., Huang,G., Gu,J., Hood,L., Rowen,L., Madan,A., Qin,S., Davis,R.W., Federspiel,N.A., Abola,A.P., Proctor,M.J., Myers,R.M., Schmutz,J., Dickson,M., Grimwood,J., Cox,D.R., Olson,M.V., Kaul,R., Raymond,C., Shimizu,N., Kawasaki,K., Minoshima,S., Evans,G.A., Athanasiou,M., Schultz,R., Roe,B.A., Chen,F., Pan,H., Ramser,J., Lehrach,H., Reinhardt,R., McCombie,W.R., de la Bastide,M., Dedhia,N., Blocker,H., Hornischer,K., Nordsiek,G., Agarwala,R., Aravind,L., Bailey,J.A., Bateman,A., Batzoglou,S., Birney,E., Bork,P., Brown,D.G., Burge,C.B., Cerutti,L., Chen,H.C., Church,D., Clamp,M., Copley,R.R., Doerks,T., Eddy,S.R., Eichler,E.E., Furey,T.S., Galagan,J., Gilbert,J.G., Harmon,C., Hayashizaki,Y., Haussler,D., Hermjakob,H., Hokamp,K., Jang,W., Johnson,L.S., Jones,T.A., Kasif,S., Kaspryzk,A., Kennedy,S., Kent,W.J., Kitts,P., Koonin,E.V., Korf,I., Kulp,D., Lancet,D., Lowe,T.M., McLysaght,A., Mikkelsen,T., Moran,J.V., Mulder,N., Pollara,V.J., Ponting,C.P., Schuler,G., Schultz,J., Slater,G., Smit,A.F., Stupka,E., Szustakowski,J., Thierry-Mieg,D., Thierry-Mieg,J., Wagner,L., Wallis,J., Wheeler,R., Williams,A., Wolf,Y.I., Wolfe,K.H., Yang,S.P., Yeh,R.F., Collins,F., Guyer,M.S., Peterson,J., Felsenfeld,A., Wetterstrand,K.A., Patrinos,A., Morgan,M.J., de Jong,P., Catanese,J.J., Osoegawa,K., Shizuya,H., Choi,S. and Chen,Y.J. CONSRTM International Human Genome Sequencing Consortium TITLE Initial sequencing and analysis of the human genome JOURNAL Nature 409 (6822), 860-921 (2001) PUBMED 11237011 REMARK Erratum:[Nature 2001 Aug 2;412(6846):565] COMMENT REFSEQ INFORMATION: The reference sequence is identical to CM000670.2. On Feb 3, 2014 this sequence version replaced NC_000008.10. Assembly Name: GRCh38.p13 Primary Assembly The DNA sequence is composed of genomic sequence, primarily finished clones that were sequenced as part of the Human Genome Project. PCR products and WGS shotgun sequence have been added where necessary to fill gaps or correct errors. All such additions are manually curated by GRC staff. For more information see: https://genomereference.org. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20200815 Annotation Version :: 109.20200815 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.5 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..18 /organism="Homo sapiens" /mol_type="genomic DNA" /db_xref="taxon:9606" /chromosome="8" ORIGIN 1 ctccctttgt tgtgttgt //
Article Source… https://pieceofmindful.com/2020/04/06/bombshell-who-coronavirus-pcr-test-primer-sequence-is-found-in-all-human-dna/
